The N-terminal 303 amino acids of the human cytomegalovirus envelope glycoprotein B (UL55) and the exon 4 region of the major immediate early protein 1 (UL123) induce a cytotoxic T-cell response

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• 作者：Berencsi ; Klara ; Gö ; nczö ; l ; Eva ; Endresz ; Valeria ; Kough ; John ; Takeda ; Shin ; Gyulay ; Zsofia ; et. al.
• 关键词：CTL epitope ; HCMV ; IE1 exon 4 ; Ad-HCMV recombinants
• 刊名：Vaccine
• 出版年：1996
• 出版时间：April, 1996
• 年：1996
• 卷：14
• 期：5
• 页码：369-374
• 全文大小：637 K

文摘

We reported earlier that an adenovirus (Ad) recombinant expressing the full-length human cytomegalovirus (HCMV) glycoprotein B (gB) gene induces gB-specific cytotoxic T lymphocyte (CTL) responses in CBA (H-2^k) mice (Berencsi et al., J. Gen. Virol.74, 257-2512, 1993). Here we show that mice immunized with Ad recombinant viruses expressing truncated forms of the gB gene containing the first 700 (Ad-700), 465 (Ad-465) or 303 (Ad-303) amino acids of gB or an Ad construct containing exon 4 (E4) of the HCMV immediate early 1 (IE1) gene (Ad-IE1 (E4)) demonstrate HCMV-specific CTL responses. These data suggest the importance of the first 303 amino acids of the gB polypeptide and the IE1 E4 product in designing a vaccine to induce anti-HCMV CTL responses.