文摘
Fagopyrin is a phototoxic substance found in buckwheat products in varying amounts. The aim of this study was to determine the fagopyrin, rutin, and quercetin contents of plant samples taken from 3 buckwheat species, namely common (Fagopyrum esculentum), Tartary (F. tataricum), and cymosum buckwheat (F. cymosum). HPLC was optimized for fagopyrin analysis using a fluorescence detector, and for other components a UV/VIS detector, while hypericin, rutin, and quercetin were used as standards. Common buckwheat leaves contained high levels of fagopyrin (322-2300 渭g g鈭?) while even higher levels were found in other samples such as the flowers (鈮?830 渭g g鈭?). The highest fagopyrin content (20,700 渭g g鈭?) was found in the flowers of F. cymosum. Two cymosum samples had a high proportion of fagopyrin relative to rutin (鈭?00 渭g fagopyrin mg鈭? rutin), whereas this proportion was lower (15-90 渭g fagopyrin mg鈭? rutin) in other samples.