DEEPN as an Approach for Batch Processing of Yeast 2-Hybrid Interactions
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- 作者：Natasha Pashkova1 ; 3 ; Tabitha A. Peterson1 ; 3 ; Venkatramanan Krishnamani1 ; Patrick Breheny2 ; Mark Stamnes1 ; Robert C. Piper1 ; 4 ; robert-piper@uiowa.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：protein interactions ; protein networks ; ubiquitin
- 刊名：Cell Reports
- 出版年：2016
- 出版时间：27 September 2016
- 年：2016
- 卷：17
- 期：1
- 页码：303-315
- 全文大小：4411 K
文摘
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Parameters of the batch Y2H approach are defined
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Data processing and statistical analysis software packages are outlined
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A DEEPN screen identifies multiple proteins that interact with ubiquitin
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A DEEPN screen differentiates proteins that bind Rab5-GTP versus Rab5-GDP