Synthesis and Characterization of a Novel ELF-97-based Fluorescent Probe for Hydrogen Peroxide Detection

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• 作者：Zi-Jun CAI ; Yong-Qing KUANG ; ^{yqkuang@hnu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Dan PAN ; Wei LIU ; Jian-Hui JIANG
• 关键词：Fluorescent probe ; 2-(2&prime ; -Hydroxy-5&prime ; -chlorophenyl)-6-chloro-4(3H)-quinazolinone ; Hydrogen peroxide
• 刊名：Chinese Journal of Analytical Chemistry
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：43
• 期：11
• 页码：1671-1675
• 全文大小：919 K

文摘

A novel 2-(2′-hydroxy-5′-chlorophenyl)-6-chloro-4(3H)-quinazolinone (ELF-97)-based fluorescent probe (P1) for H₂O₂ analysis was prepared with 5-chlorosalicylaldehyde, 4-(bromomethyl)phenylboronic acid and 2-amino-5-chlorobenzamide as the materials, and its structure was characterized by ¹H nuclear magnetic resonance (NMR), ¹³C NMR and high resolution mass spectrometry (HRMS). Weak fluorescence intensity was observed at 425 nm when the solution of probe P1 was excited by 360 nm UV light. After addition of H₂O_2, however, the emission peak at 425 nm disappeared while a strong emission peak at 515 nm appeared with the same excitation wavelength (360 nm). The fluorescence intensity at 515 nm is dependent on the concentration of H₂O_2, with the linear response range of 5–45 μM. The detection limit and the recovery of H₂O₂ in milk sample were 0.1 μM and 94.0%–106.0%, respectively. The results show that probe P1 is potential to become a useful tool for rapid detection of H₂O₂.