Alleviating anastrozole induced bone toxicity by selenium nanoparticles in SD rats

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• 作者：Kiritkumar K. Vekariya ; Jasmine Kaur ; Kulbhushan Tikoo ; ^{tikoo.k@gmail.com}
• 关键词：SeNPs ; Selenium nanoparticles ; AI ; Aromatase Inhibitors ; ALKP ; Alkaline Phosphatase ; TRAP ; Tartrate Resistant Acid Phosphatase ; OVR ; Ovariectomized rats ; MTT ; 3-(4 ; 5-Dimethylthiazal-2-yl)-2 ; 5-diphenyltetrazolium bromide
• 刊名：Toxicology and Applied Pharmacology
• 出版年：2013
• 出版时间：15 April, 2013
• 年：2013
• 卷：268
• 期：2
• 页码：212-220
• 全文大小：1698 K

文摘

Aromatase inhibitors like anastrozole play an undisputed key role in the treatment of breast cancer, but on the other hand, various side effects like osteoporosis and increased risk of bone fracture accompany the chronic administration of these drugs. Here we show for the first time that selenium nanoparticles, when given in conjugation to anastrozole, lower the bone toxicity caused by anastrozole and thus reduce the probable damage to the bone. Selenium nanoparticles at a dose of 5 ¦Ìg/ml significantly reduced the cell death caused by anastrozole (1 ¦ÌM) in HOS (human osteoblast) cells. In addition, our results also highlighted that in female SD rat model, SeNPs (0.25, 0.5, 1 mg/kg/day) significantly prevented the decrease in bone density and increase in biochemical markers of bone resorption induced by anastrozole (0.2 mg/kg/day) treatment. Histopathological examination of the femurs of SeNP treated group revealed ossification, mineralization, calcified cartilaginous deposits and a marginal osteoclastic activity, all of which indicate a marked restorative action, suggesting the protective action of the SeNPs. Interestingly, SeNPs (1 mg/kg/day) also exhibited protective effect in ovariectomized rat model, by preventing osteoporosis, which signifies that bone loss due to estrogen deficiency can be effectively overcome by using SeNPs.