spara20">ClustalW analysis for phylogram, BLASTP analysis for E and NS proteins of DENV serotypes and Swiss-Prot for 3D structure of E and NS proteins were used.
spara30">E proteins were grouped into two clusters, DENV-1, DENV-2 and DENV-3 forming one cluster and DENV-4 alone forming a different serotype. On the surfaces of E proteins of four serotypes, five binding sites for ligands were located in different DENV which the fourth binding site was identified as the active ligand binding site in DENV-2, DENV-3 and DENV-4 serotypes and second binding site in the DENV-1. Among the seven NS proteins, NS-1, NS-3 and NS-5 were identified as significant molecules involved in dengue disease.
spara40">Binding sites, essential for ligand binding are found on all the E and NS proteins which active binding sites have been identified.