Cells were prepared from rats stably expressing GCaMP2 Ca2 + sensor protein. G5 induced Ca2 + oscillation and sustained Ca2 + signals at 1 and10 μM in hepatocytes. Activation of Kupffer cells by LPS and G5 decreased the inducibility of CYP2B1/2. G5 reduced the canalicular efflux of bilirubin in co-cultures. G5 stimulated the uptake and canalicular efflux of taurocholate.