Developmental expression of canalicular transporter genes in human liver

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• 作者：Huey-Ling Chen ; Hui-Ling Chen ; Yu-Jung Liu ; Chun-Hsiang Feng ; Chao-Yi Wu ; Ming-Kwang Shyu ; Ray-Hwang Yuan and Mei-Hwei Chang
• 刊名：Journal of Hepatology
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：43
• 期：3
• 页码：472-477
• 全文大小：263 K

文摘

Background/Aims

BSEP, MRP2, and MDR3 are major hepatic canalicular transporters mediating bile secretion. Their expression in human liver during development has not been reported.

Methods

Human liver samples from fetus at gestational age 14–20 weeks, adult livers and liver samples of infants with biliary atresia were tested for mRNA expression of BSEP, MDR3, MRP2, NTCP, FIC1, and FXR genes by using real-time RT-PCR. Immunohistochemical staining of BSEP, MDR3, and MRP2 were performed on fetal and adult livers.

Results

All the genes tested were expressed at mid-gestational age. MDR3 and NTCP showed significant lower levels in fetal livers compared to adults. In patients with biliary atresia, all the genes tested showed higher mean expression levels than adults except for NTCP, but not statistically significant. The immunohistochemical staining of MRP2 in fetal liver was canalicular, BSEP showed both intracellular and canalicular staining, and MDR3 staining was faint, only occasional canalicular pattern could be seen.

Conclusions

The major canalicular transporter genes are expressed at mid-gestational stage during human fetal development, but are different in expression level and targeting pattern, indicating differential regulation and maturation.