Direct electrochemistry of cytochrome c with three-dimensional nanoarchitectured multicomponent composite electrode and nitrite biosensing

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• 作者：Komathi Shanmugasundaram^a ; ¹Author Vitae ; Gopalan Sai-Anand^b ; ¹Author Vitae ; Anantha-Iyengar Gopalan^cAuthor Vitae ; Hyun-Gyu Lee^dAuthor Vitae ; Ho Kwon Yeo^aAuthor Vitae ; Shin-Won Kang^b ; ^{swkang@knu.ac.kr" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Kwang-Pill Lee^a ; ^c ; ^{kplee@knu.ac.kr" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Cytochrome c ; Nanoarchitecture ; Multicomponent ; Nitrite
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：2 June 2016
• 年：2016
• 卷：228
• 期：Complete
• 页码：737-747
• 全文大小：2271 K

文摘

A novel electrochemical nitrite ion (NO₂⁻) biosensor was designed and fabricated by modifying a glassy carbon electrode (GCE) into three-dimensional nanoarchitectured electrode (3DNE) using a multicomponent nanocomposite (MCNC) film composed of graphene embedded titanium dioxide nanowires (TiO₂(G) NWs), thiol-functionalized polyaniline (PANI(SH)), gold nanoparticles (Au), and immobilized cytochrome c (cyt c). The new NO₂⁻ biosensor was designated as cyt c/TiO₂(G) NWs@PANI(SH)-Au MCNC/3DNE. The assembly of the 3DNE, involved sequential depositions of PANI(SH) and Au over previously synthesized TiO₂(G) NWs by electrospinning-hydrothermal processes and immobilization of the cyt c onto the TiO₂(G) NWs/PANI(SH)/Au MCNC film. The cyt c/TiO₂(G) NWs@PANI(SH)-Au MCNC/3DNE exhibited direct electron transfer from cyt c to the electrode at a high rate constant (25.34 s⁻¹). The fabricated cyt c/TiO₂(G) NWs@PANI(SH)-Au MCNC/3DNE showed high selectivity to NO₂⁻ ions with excellent sensitivity (9.2 μA/mM), a wide linear concentration range (10 μM–720 mM), and a low detection limit (0.05 μM). Additionally, the cyt c/TiO₂(G) NWs@PANI(SH)-Au MCNC/3DNE exhibited high stability with good reproducibility and repeatability.