Putative prognostic epithelial-to-mesenchymal transition biomarkers for aggressive prostate cancer

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• 作者：Helen Whitel ; Samantha Spencer-Harty ; David Hywel Thomas ; Christine Davies ; Claire Morgan ; Howard Kynaston ; Pradeep Bose ; Neil Fenn ; Paul D. Lewis ; Owen Bodger ; Spencer Jenkins ; Shareen H. Doak
• 关键词：Prostate cancer ; Epithelial-to-mesenchyme transition ; E-Cadherin ; Snail ; Gleason score ; Clinical stage
• 刊名：Experimental and Molecular Pathology
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：95
• 期：2
• 页码：220-226
• 全文大小：1362 K

文摘

Prostate cancer is the second most frequently diagnosed cancer worldwide and is the sixth leading cause of cancer deaths in men, yet it varies greatly in its aggressiveness. Currently, it is not possible to adequately differentiate between patients whose tumors will remain indolent and those patients whose disease will progress, resulting in unnecessary aggressive treatment. Consequently, there is an urgent need to identify markers of prostate cancer progression, invasiveness and metastasis to more accurately predict prognosis. The aim of this study was to assess the ability of key epithelial-to-mesenchymal transition molecules in identifying prostate cancer patients who are likely to develop aggressive tumors. Using 215 archival patient tissue samples, immunohistochemistry was applied to examine the expression and sub-cellular localization of E-Cadherin, Snail, Slug, Twist, Vimentin, BMP-2 and BMP-7. Of the seven markers assessed, a significantly increased expression of Snail protein was observed within the nucleus of prostate cancer cells and was strongly associated with increasing Gleason score and clinical stage. In addition, loss of E-Cadherin expression at the cellular membrane of prostate cancer cells was also significantly associated with increasing Gleason score, clinical stage, and additionally, a reduction in survival.