Determination of thermodynamic parameters of benzylpenicillin hydrolysis by metallo-¦Â-lactamase CcrA from Bacteroides fragilis

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• 作者：Le Zhai ; Li-Sheng Zhou ; Cheng-Cheng Liu ; Ying Shi ; Ya-Jun Zhou ; Ke-Wu Yang ; ^{kwyang@nwu.edu.cn}
• 关键词：Antibiotic resistance ; Metallo-¦Â-lactamase CcrA ; Thermodynamic parameters ; Microcalorimetry
• 刊名：Thermochimica Acta
• 出版年：2013
• 出版时间：20 March, 2013
• 年：2013
• 卷：556
• 期：Complete
• 页码：54-57
• 全文大小：219 K

文摘

One of the most common way that bacteria become resistant to antibiotics is by the production of metallo-¦Â-lactamases (M¦ÂLs) to hydrolyze the ¦Â-lactam-containing antibiotics. In this paper, the thermodynamic parameters of benzylpenicillin hydrolysis with B1 subclasses M¦ÂL CcrA (carbapenem and cephamycin resistance) from Bacteroides fragilis were determined by microcalorimetry. The activation free energy is 87.90 ¡À 0.03, 88.99 ¡À 0.01, 89.93 ¡À 0.04 and 90.93 ¡À 0.05 kJ mol^?1 at 293.15, 298.15, 303.15 and 308.15 K, activation enthalpy is 29.21 ¡À 0.03 kJ mol^?1, activation entropy is ?200.34 ¡À 0.08 J mol^?1 K^?1, the reaction order is 1.4, and the apparent activation energy E is 31.71 kJ mol^?1. The thermodynamic characterization indicated that CcrA prefer to hydrolyze penicillins among three kinds of ¦Â-lactam-containing antibiotics.