Investigating the position of the hairpin loop in New Delhi metallo-β-lactamase, NDM-1, during catalysis and inhibitor binding
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- 作者：Mahesh Aithaa ; Abraham J. Mollera ; Indra D. Sahua ; Masaki Horitanib ; David L. Tierneya ; Michael W. Crowdera ; crowdemw@miamioh.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：DEER spectroscopy ; Rapid freeze quench ; Metallo-β-lactamase ; Site specific spin labeling
- 刊名：Journal of Inorganic Biochemistry
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：156
- 期：Complete
- 页码：35-39
- 全文大小：780 K
文摘
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We used double electron electron resonance (DEER) spectroscopy to study metallo-β-lactamase.
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We used rapid-freeze DEER studies to probe loop motions during catalysis.
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Loop motions in New Delhi metallo-β-lactamase, NDM-1, were evaluated.
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One reaction was freeze quenched at 500 μs.
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Product binding does not affect the position of the loop.