Analysis of antibiotics in urine and wipe samples from environmental and biological monitoring—Comparison of HPLC with UV-, single MS- and tandem MS-detection

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• 作者：Jochen Tuerk ; Marius Reinders ; Dennis Dreyer ; Thekla K. Kiffmeyer ; Klaus Gerhard Schmidt and Heinz-Martin Kuss
• 关键词：Liquid chromatography-mass spectrometry ; Antibiotics ; Sulfonamides ; β ; -Lactames ; Fluoroquinolones ; Wipe Samples ; Urine ; Environmental and biological monitoring ; Occupational exposure
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2006
• 出版时间：2 February 2006
• 年：2006
• 卷：831
• 期：1-2
• 页码：72-80
• 全文大小：181 K

文摘

Results of the simultaneous determination of the structurally different antibiotics cefazoline, cefotiame, cefuroxime, chloramphenicol, ciprofloxacin, ofloxacin, sulfamethoxazole and trimethoprim from environmental and biological monitoring using high-performance liquid chromatography with UV, single mass and tandem mass spectrometry were compared. For sample enrichment and clean-up a SPE method using bakerbond C18 cartridges was developed. Mean recovery rates were above 70 % . Because of the complex urine matrix, only the wipe samples could be analyzed by UV-detection. However, UV-detection and single MS-detection are useful for control measurements after spillage, e.g. (LOD = 1–2 ng/cm²). Samples from biological monitoring of occupational uptake should be analyzed by LC–MS/MS. The limits of detection (LOD) in urine ranged from 0.4 to 70 μg/L for LC–MS and 0.01 to 0.9 μg/L for LC–MS/MS detection. The limits of detection in wipe samples ranged from 0.003 to 0.13 ng/cm².