Direct-immersion solid-phase microextraction coupled to fast gas chromatography mass spectrometry as a purification step for polycyclic aromatic hydrocarbons determination in olive oil

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• 作者：Giorgia Purcaro ; Massimo Picardo ; Laura Barp ; Sabrina Moret ; Lanfranco S. Conte
• 关键词：Polycyclic aromatic hydrocarbons (PAHs) ; Solid-phase microextraction (SPME) ; Direct immersion (DI) ; Zirconia-based sorbent (Z-Sep+) ; Gas chromatography&ndash ; mass spectrometry (GC&ndash ; MS) ; Edible oil
• 刊名：Journal of Chromatography A
• 出版年：2013
• 出版时间：13 September, 2013
• 年：2013
• 卷：1307
• 期：Complete
• 页码：166-171
• 全文大小：1318 K

文摘

The aim of the present work was to optimize a preparation step for polycyclic aromatic hydrocarbons in a fatty extract. Solid-phase microextraction is an easy preparation technique, which allows to minimize solvent consumption and reduce sample manipulation. A Carbopack Z/polydimethylsiloxane fiber, particularly suitable for extraction of planar compounds, was employed to extract polycyclic aromatic hydrocarbons from a hexane solution obtained after a previous extraction with acetonitrile from oil, followed by a liquid-liquid partition between acetonitrile and hexane. The proposed method was a rapid and sensitive solution to reduce the interference of triglycerides saving the column life and avoiding frequent cleaning of the mass spectrometer ion source. Despite the non-quantitative extraction of polycyclic aromatic hydrocarbons from oil using acetonitrile, the signal-to-noise ratio was significantly improved obtaining a limit of detection largely below the performance criteria required by the European Union legislation.