In thi
s paper, lipa
se activity wa
s characterized in coffee (
Coffea arabica L.)
seed
s to determine it
s involvement in lipid degradation during germination. The lipa
se activity, evaluated by a colorimetric method, wa
s already pre
sent before imbibition of
seed
s and wa
s further induced during the germination proce
ss. The activity
showed a bipha
sic behaviour, which wa
s similar in
seed
s either with or without endocarp (parchment), even though the phenomenon
showed a delay in the former. The enzymatic activity wa
s inhibited by tetrahydrolip
statin (THL), a
selective and irrever
sible inhibitor of lipa
se
s, and by a polyclonal antibody rai
sed again
st purified alkaline lipa
se from ca
stor bean.
The immunochemical analysis evidenced a protein of ca. 60 kDa, cross-reacting with an anti-lipase antibody, in coffee samples obtained from seeds of both types. Gas chromatographic analyses of free fatty acid (FFA) content confirmed the differences shown in the lipolytic activity of the samples with or without parchment, since FFA levels increased more rapidly in samples without parchment. Finally, the analyses of the antioxidant capacity showed that the presence of parchment was crucial for lowering the oxidation of the lipophylic fraction, being the seeds with parchment less prone to oxidation processes.