Dansylation isotope labeling liquid chromatography mass spectrometry for parallel profiling of human urinary and fecal submetabolomes
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- 作者：Xiaoling Sua ; Nan Wanga ; b ; Deying Chena ; Yunong Lib ; Yingfeng Lua ; Tao Huanb ; Wei Xua ; Liang Lia ; b ; Liang.Li@ualberta.ca" class="auth_mail" title="E-mail the corresponding author ; Lanjuan Lia ; ljli@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Mass spectrometry ; Liquid chromatography ; Isotope labeling ; Urine ; Feces ; Metabolomics
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：903
- 期：Complete
- 页码：100-109
- 全文大小：1093 K
文摘
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A method of parallel analysis of urine and fecal metabolomes is developed.
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Dansylation isotope labeling LC-MS is used for amine/phenol submetabolome quantification.
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Detection of 3089 metabolites in urine and 3012 metabolites in feces for a total of 5372 metabolites.
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Parallel profiling of urine and feces increases the metabolomic coverage.