Large fragment deletion using a CRISPR/Cas9 system in Saccharomyces cerevisiae

详细信息    查看全文

• 作者：Huanhuan Hao ; Xiaofei Wang ; Haiyan Jia ; Miao Yu ; Xiaoyu Zhang ; Hui Tang ; Liping Zhang ; ^{zhanglphbu@sohu.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Large-fragments deletion ; Latour system ; CRISPR/Cas9 ; Comparation ; Saccharomyces cerevisiae
• 刊名：Analytical Biochemistry
• 出版年：2016
• 出版时间：15 September 2016
• 年：2016
• 卷：509
• 期：Complete
• 页码：118-123
• 全文大小：828 K

文摘

Large chromosomal modifications have been performed in natural and laboratory evolution studies and hold tremendous potential for use in foundational research, medicine, and biotechnology applications. Recently, the type II bacterial Clustered Regularly Interspaced Short Palindromic Repeat and CRISPR-associated (CRISPR/Cas9) system has emerged as a powerful tool for genome editing in various organisms. In this study, we applied the CRISPR/Cas9 system to preform large fragment deletions in Saccharomyces cerevisiae and compared the performance activity to that of a traditional method that uses the Latour system. Here we report in S. Cerevisiae the CRIPR/Cas9 system has been used to delete fragments exceeding 30 kb. The use of the CRISPR/Cas9 system for generating chromosomal segment excision showed some potential advantages over the Latour system. All the results indicated that CRISPR/Cas9 system was a rapid, efficient, low-cost, and versatile method for genome editing and that it can be applied in further studies in the fields of biology, agriculture, and medicine.