Molecular cloning and characterization of LPS-binding protein/bactericidal permeability-increasing protein (LBP/BPI) from olive flounder, Paralichthys olivaceus
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- 作者：Bo-Hye Nam ; Kyoung Jin Ahn ; Young-Ok Kim ; Hee Jeong Kong ; Woo-Jin Kim ; Hyung Soo Kim ; Sang-Jun Lee ; Kyong Kil Kim
- 关键词：LPS ; LBP/BPI ; Olive flounder ; Paralichthys olivaceus
- 刊名：Veterinary Immunology and Immunopathology
- 出版年：2010
- 出版时间：15 February 2010
- 年：2010
- 卷：133
- 期：2-4
- 页码：256-263
- 全文大小：1322 K
文摘
A lipopolysaccharide (LPS)-binding protein/bactericidal permeability-increasing protein (LBP/BPI) homolog was isolated from peripheral blood leukocytes cDNA library of olive flounder Paralichthys olivaceus. The isolated LBP/BPI cDNA is 2806 bp in length with a 1419 bp open reading frame (ORF) that encodes a protein of 472 amino acid residues. The LPS-binding domain is well conserved in the N-terminal barrel, showing high sequence identities with other teleost LBP/BPI as well as those of mammals. RT-PCR analysis revealed that mRNA expression of LBP/BPI was significantly elevated in all tested tissues (liver, gill, intestine, head kidney, and spleen) after intraperitoneal injection of the gram-negative bacterium Edwardsiella tarda or the gram-positive bacterium Streptococcus iniae. This expression pattern profile corresponded to that of acute inflammatory cytokines, suggesting that it plays a role in the innate immune response, in particular, the acute phase response.