Primary vaccination with the LiESP/QA-21 vaccine (CaniLeish^庐) produces a cell-mediated immune response which is still present 1 year later

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• 作者：Javier Moreno^a ; Ioannis Vouldoukis^b ; Paul Schreiber^c ; Virginie Martin^c ; David McGahie^d ; ^{david.mcgahie@virbac.com" class="auth_mail} ; Sylvie Gueguen^c ; Anne-Marie Cuisinier^c
• 关键词：BrdU ; 5-bromo-2-deoxyuridine ; CMLA ; Canine Macrophage Leishmanicidal Assay ; ELISpot ; Enzyme-Linked Immunospot Assay ; IFAT ; immunofluorescent antibody test ; iNOS ; inducible nitric oxide synthase ; LiESP ; L. infantum excreted-secreted proteins ; LTT ; Lymphoblastic Transformation Test ; NO ; nitric oxide ; PSA ; parasite surface antigen ; SLA ; Soluble Leishmania Antigens
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：15 April, 2014
• 年：2014
• 卷：158
• 期：3-4
• 页码：199-207
• 全文大小：903 K

文摘

Canine leishmaniasis, an important zoonotic disease of dogs, is the result of an ineffective and inappropriate immune response to infection with Leishmania infantum. It is widely accepted that the appropriate immune response is characterised by a T-helper (Th)1-dominated profile in an overall mixed Th1/Th2 response. The absence of a strong Th1 response is associated with progression to the clinical disease. Thus, there is a need for an effective vaccine that could modulate the immune response to a more appropriate profile against the parasite. In this study we measured the impact of the LiESP/QA-21 canine vaccine, recently launched commercially in Europe, on selected humoral and cellular immune markers for one year after a primary vaccination course. The humoral response to vaccination was characterised by a predominantly IgG2 profile. Vaccinated dogs developed long-lasting cell-mediated immune responses against L. infantum, specifically with a stronger ability of macrophages to reduce intracellular parasite burdens in co-culture with autologous lymphocytes compared to control dogs (p = 0.0002), which was correlated with induction of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO) derivatives. These results confirm that vaccination with LiESP/QA-21 is capable of inducing an appropriate Th1-dominated immune profile which persists for a full year.