Protective inflammatory response against visceral leishmaniasis with potato tuber extract: A new approach of successful therapy

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• 作者：Dibyendu Paik^a ; Partha Das^a ; Kshudiram Naskar^b ; Pijush Kanti Pramanik^a ; Tapati Chakraborti ; PhD Dr.^a ; ^{tcbiochem@gmail.com" class="auth_mail" title="E-mail the corresponding author} (Professor)
• 关键词：PTEx ; potato tuber extract ; NO ; nitric oxide ; ROS ; reactive oxygen species ; VL ; visceral leishmaniasis ; CL ; cutaneous leishmaniasis ; MCL ; mucocutaneous leishmaniasis ; LPS ; lipopolysaccharide ; DMSO ; dimethyl sulfoxide ; SAG ; sodium antimony gluconate ; PBS ; phosphate buffer saline ; H2DCFDA ; 2&prime ; 7&prime ; -dichlorodihydrofluorescein diacetate ; DAF-2DA ; 4 ; 5-diaminofluorescein diacetate ; HGPRT ; hypoxanthin &ndash ; eguanine phosphoribosyl transferase ; SAG ; Soduim antimony gluconate
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：83
• 期：Complete
• 页码：1295-1302
• 全文大小：1282 K

文摘

The increasing number of drug resistance issue of Leishmania donovani strain to common drugs compels to develop new therapeutics against leishmaniasis with minimal toxicity. In this regard, bioactive phytocomponents may lead to the discovery of new medicines with appropriate efficiency. The important roles of Leishmania proteases in the virulence of Leishmania parasite make them very hopeful targets for the improvement of current remedial of leishmaniasis. As part of a hunt for new drugs, we have evaluated in vivo anti-leishmanial activity of serine protease inhibitor rich fraction (PTEx), isolated by sodium bisulfite extraction from potato tuber. The amastigote load of 25 mg/kg body weight/day treated BALB/c mice showed 86.9% decrease in liver and 88.7% in case of spleen. This anti-leishmanial effect was also supported by PTEx induced immunomodulatory activity like acute formation of ROS and prolonged NO generation. The Th1/Th2 cytokine balance in splenocytes of PTEx treated animals was estimated and evaluated by ELISA assay as well as by mRNA expression using RT-PCR. Furthermore, significant survival rate (80%) was observed in PTEx treated hamsters. Thus, from the present observations we could accentuate the potential of PTEx to be employed as a new therapeutics from natural source against L. donovani. This might also provide a novel perception of natural serine protease inhibitor from potato tuber as an alternate approach for the treatment of visceral leishmaniasis.