A novel dendritic cell-targeted lentiviral vector, encoding Ag85A-ESAT6 fusion gene of Mycobacterium tuberculosis, could elicit potent cell-mediated immune responses in mice
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- 作者：Mehdi Shakouria ; Seyed Mohammad Moazzenib ; moazzeni@modares.ac.ir" class="auth_mail" title="E-mail the corresponding author ; Mostafa Ghaneic ; mghanei@hbi.ir" class="auth_mail" title="E-mail the corresponding author ; Arash Arashkiad ; a_arashkia@pasteur.ac.ir" class="auth_mail" title="E-mail the corresponding author ; Mohammad Hossein Etemadzadehd ; Kayhan Azadmaneshd ; azadmanesh@pasteur.ac.ir" class="auth_mail" title="E-mail the corresponding author
- 关键词：Tuberculosis ; Dendritic cell ; DC-targeted lentivector ; Cellular immunity
- 刊名：Molecular Immunology
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：75
- 期：Complete
- 页码：101-111
- 全文大小：1980 K
文摘
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We developed a novel DC-directed lentivector, LV-GFP/SVGmu that could specifically transduce DC-SIGN-expressing cells with a high efficiency.
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The DC- directed lentivector, LV-AEG/SVGmu, was also able to successfully transduce and express the fusion antigen Ag85A-E6-GFP in DC-SIGN-expressing cells.
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A single footpad injection of LV-AEG/SVGmu could elicit significant elevated cellular and humoral immune responses in mice.
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Hence, it can be considered as a novel candidate for vaccine research and immunotherapy in future.