Purification and partial characterisation of a cathepsin L-like proteinase from sea cucumber (Stichopus japonicus) and its tissue distribution in body wall

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• 作者：Da-Yong Zhou^a ; ^b ; Xian-Na Chang^a ; ^b ; Sha-Sha Bao^a ; Liang Song^a ; ^b ; Bei-Wei Zhu^a ; ^b ; ^{zhubeiwei@163.com" class="auth_mail} ; Xiu-Ping Dong^a ; ^b ; Yuan Zong^a ; ^b ; Dong-Mei Li^a ; ^b ; Mao-Mao Zhang^a ; ^b ; Yu-Xin Liu^a ; ^b ; Yoshiyuki Murata^c
• 关键词：Sea cucumber (Stichopus japonicus) ; Body wall ; Cathepsin L-like proteinase ; Autolysis ; Immunohistochemistry
• 刊名：Food Chemistry
• 出版年：1 September, 2014
• 年：2014
• 卷：158
• 期：Complete
• 页码：192-199
• 全文大小：875 K

文摘

A cathepsin L-like proteinase (CLP) with molecular weight of 30.9 kDa from the gut of sea cucumber (Stichopus japonicas, S. japonicus) was isolated and purified to homogeneity by several chromatographic procedures. The enzyme exhibited optimum activity at pH 5.0-5.5 and 50 掳C, and showed thermostability up to 40 掳C. The enzyme activity was completely inhibited by Zn²⁺, strongly inhibited by Fe²⁺ and Cu²⁺, drastically reduced by cysteine proteinase inhibitors, but slightly enhanced by thiol-activating agents. The enzyme efficiently hydrolysed the specific substrate of cathepsin L, but hardly hydrolysed the specific substrates for cathepsin B, cathepsin H and cathepsin K. Immunohistochemical studies indicated that the CLP was more abundant in the epidermis rather than in the dermis of S. japonicus body wall. The distribution of CLP showed positive correlation with autolysis rate. Therefore, the relationship between CLP and autolysis deserved further study.