An acute rejection model of liver transplantation was established in inbred rats DA to LEW that were randomly divided into a control group and a LXA4 group. Liver morphologic changes were examined using hematoxylin/eosin staining. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were quantified to measure liver injury. Intragraft mRNA and protein expressions of interferon (IFN)-¦Ã interleukin and (IL)-10 were detected by real-time polymerase chain reaction and Western blots, respectively. The serum levels of IFN-¦Ã and IL-10 were assayed by enzyme-linked immunosorbent assay.
LXA4 treatment improved hepatic tissue injury as indicated by morphologic analysis. Serum ALT and AST levels were significantly decreased at day 7 post-transplantation (P?< .05). Concurrently, expression of IFN-¦Ã was downregulated (P?< .05) and secretion of IL-10 was enhanced (P?< .05).
LXA4 attenuated acute rejection with a parallel shift from Th1 to Th2 responses in rat liver transplantation.