Monosialoganglioside-GM1 triggers binding of the amyloid-protein salmon calcitonin to a Langmuir membrane model mimicking the occurrence of lipid-rafts
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- 作者：Marco Diociaiutia ; marco.diociaiuti@iss.it" class="auth_mail" title="E-mail the corresponding author ; Cristiano Giordania ; b ; Gihan S. Kamelc ; d ; Francesco Brasilid ; Simona Sennatod ; Cecilia Bombellie ; Karen Y. Menesesb ; Marco A. Giraldob ; Federico Bordid
- 关键词：Membrane Models ; Lipid-rafts ; GM1 ; Amyloid ; sCT ; Langmuir Films ; AFM ; ζ-potential ; BAM
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：8
- 期：Complete
- 页码：365-375
- 全文大小：1218 K
文摘
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Membrane models mimicking the occurrence of lipid-rafts and sCT, were investigated.
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Atomic Force Microscopy shows sCT globular aggregates onto very flat surfaces.
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ξ-potential data indicate that electrostatic interaction occurs between sCT and GM1.
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Thermodynamic data of the outer leaflet reveal that sCT aggregate outside the film.
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GM1, one component of the lipid-rafts, result to trigger the interaction.