Depletion of CD147 sensitizes human malignant melanoma cells to hydrogen peroxide-induced oxidative stress

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• 作者：Ji Li ; Lefang Peng ; Lisha Wu ; Yehong Kuang ; Juan Su ; Mei Yi ; Xinglin Hu ; Dai Li ; Hongfu Xie ; Takuro Kanekura ; Xiang Chen
• 刊名：Journal of Dermatological Science
• 出版年：2010
• 出版时间：June 2010
• 年：2010
• 卷：58
• 期：3
• 页码：204-210
• 全文大小：580 K

文摘

Background

Increased sensitivity to reactive oxygen species (ROS) contributes to the effectiveness of therapeutic strategies in patients with malignant melanoma (MM). CD147, a cell surface receptor for cyclophilin A (CypA), is thought to exert antioxidant activities.

Objective

To understand the influences and mechanisms of CD147 on proliferation, apoptosis and redox state of A375 cells under H₂O₂-induced oxidative stress.

Methods

The effect of CD147 silencing on cell viability, apoptosis, the generation of ROS, superoxide dismutase (SOD) activity, and the malondialdehyde (MDA) level that reflects oxidative damage, was measured in human malignant melanoma cell line A375 treated or untreated with hydrogen peroxide (H₂O₂).

Results

In A375 cells, CD147 silencing increased the H₂O₂-induced inhibition of cell viability, H₂O₂-induced apoptosis, H₂O₂-mediated ROS- and MDA generation, and the H₂O₂-triggered decrease in SOD activity.

Conclusions

Our results demonstrated that CD147 silencing increased cellular ROS and destroyed the intrinsic antioxidant defenses in A375, indicating that CD147 exerts a cytoprotective effect against H₂O₂-induced oxidative damage.