cNGR conjugated poly(lactic acid)-poly(ethylene glycol) nanoparticles for targeted gene delivery

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• 作者：Chunxi Liu ^{liuchunxi2007@yahoo.com.cn} ; Wangyang Yu ; Zhijin Chen ; Jian Zhang ; Na Zhang
• 关键词：Gene therapy ; Active targeting ; Poly(lactic acid)-poly(ethylene glycol) ; Asparagines-glycine-arginine
• 刊名：Journal of Controlled Release
• 出版年：2011
• 出版时间：30 November, 2011
• 年：2011
• 卷：152
• 期：supp_S1
• 页码：e155-e157
• 全文大小：1K

文摘

Cyclic asparagines-glycine-arginine (cNGR) peptide was used to target gene loaded poly(lactic acid)-poly(ethylene glycol) nanoparticles (PLA-PEG NPs) to human umbilical vein endothelial cells (HUVEC). cNGR modified gene loaded PLA-PEG nanoparticles (cNGR-PLA-PEG NPs) could effectively condense DNA into small-sized complexes (< 200 nm) with positive charge (~ 10 mV) using a novel cationic surfactant, 6-lauroxyhexyl lysinate (LHLN). cNGR-PLA-PEG NPs could be internalized into HUVEC by caveolae-mediated endocytosis with higher gene transfection efficiency and lower cytotoxicity than PLA-PEG NPs.