• 作者：Isidro Machado ; Francesco Mecci ; Mar¨ªa Dolores Linares Latorre ; Carmen Illueca ; Victor Trav¨¦s ; Isabel Pic¨®n Roig ; Pablo Lorente Alegre ; Zaida Garc¨ªa-Casado ; Beatriz Llombart ; Stefano Pileri
• 关键词：Linfoma de Hodgkin ; Monoclonalidad T y B ; Linfoma compuesto
• 刊名：Revista Espa?ola de Patolog¨ªa
• 出版年：2012
• 出版时间：July-September, 2012
• 年：2012
• 卷：45
• 期：3
• 页码：186-192
• 全文大小：1743 K

Introduction

The diagnosis and management of malignant lymphoma should be based on clinical, morphological, immunohistochemical and molecular information. Particularly in cases with morphological and/or immunohistochemistry overlapping, molecular biology provides important diagnostic clues.

Material and methods

A 72 year-old male with mycosis fungoides, pancitopenia and hepatosplenomagly without lymph node enlargement, underwent both hepatic and bone marrow biopsies and histopathological, immunohistochemical and molecular studies were performed.

Results

The bone marrow and hepatic biopsies showed two different populations, one composed of large cells including typical Reed-Sternberg cells and their variant, with expression of CD30, CD15, MUM1 and EBV. The other was more extensive and revealed polymorphic medium to small cells with convoluted nuclei positive for CD3, CD4, CD2, CD7. Clonal T cell receptor gamma and monoclonal immunoglobulin H gene were detected in the bone marrow infiltration.

Conclusion

The interpretation of the molecular results in the diagnosis of lymphoma should be strictly correlated with the clinical evolution and the morphological and immunohistochemical findings.