Fumonisin B₁-induced toxicity and oxidative damage in U-118MG glioblastoma cells

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• 作者：Stockmann-Juvala ; Helene ; Mikkola ; Jouni ; Naarala ; Jonne ; Loikkanen ; Jarkko ; Elovaara ; Eivor ; et. al.
• 关键词：Fumonisin B₁ ; U-118MG glioblastoma cells ; Reactive oxygen species ; Lipid peroxidation ; Glutathione ; Apoptosis
• 刊名：Toxicology
• 出版年：2004
• 出版时间：October 1, 2004
• 年：2004
• 卷：202
• 期：3
• 页码：173-183
• 全文大小：219 K

文摘

The mycotoxin fumonisin B₁ (FB₁) is produced by Fusarium verticillioides, which commonly infects corn and other agricultural products. Fusarium species are also a frequent finding in moisture-damaged buildings, causing possible human exposure to FB₁. FB₁ is neurotoxic and carcinogenic in a number of animal species. In this study, we have investigated the effects of FB₁ on human U-118MG glioblastoma cells. The production of reactive oxygen species (ROS), lipid peroxidation, intracellular reduced glutathione (GSH) levels, cell viability, caspase-3-like protease activity and DNA fragmentation were studied in cells exposed to 0.01–100μM FB₁ for 0.5–144h. FB₁ increased lipid peroxidation and the production of ROS in U-118MG cells, showing significant effects after culture times from 48 to 144h at dose levels of 10 or 100μM FB₁. These effects were accompanied by changes in the GSH levels and cell viability, which decreased significantly after incubating the cells for 48–144h with the toxin. Signs of apoptosis were indicated by increased caspase-3-like protease activity and internucleosomal DNA fragmentation. Thus, oxidative stress and apoptosis may be involved in the neurotoxicity induced by FB₁.