Cu²⁺-selective ¡°Off-On¡± chemsensor based on the rhodamine derivative bearing 8-hydroxyquinoline moiety and its application in live cell imaging

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• 作者：Jiaoliang WangAuthor Vitae ; Liping Long ; ^{llping401@163.com}Author Vitae ; Dan XieAuthor Vitae ; Xiaofeng SongAuthor Vitae
• 关键词：Fluorescence chemsensor ; Copper(II) ; Binding ; Imaging
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：177
• 期：Complete
• 页码：27-33
• 全文大小：951 K

文摘

A rhodamine-based fluorescence chemsensor bearing the 8-hydroxyquinoline unit is developed as a reversible turn-on chemosensor for Cu²⁺. It exhibits a highly sensitive fluorescent response toward Cu²⁺ in aqueous media with an 80-fold fluorescence intensity enhancement under 10 equiv. of Cu²⁺ added. This indicates that the synthesized chemosensor effectively avoided the fluorescence quenching for the paramagnetic nature of Cu²⁺ via its strong binding capability toward Cu²⁺. The chemosensor Rh-Q exhibits a dynamic response range for Cu²⁺ from 2 ¡Á 10^?7 to 5 ¡Á 10^?5 M, with a detection limit of 0.19 ¦ÌM, and good selectivity for Cu²⁺ over other heavy and transition metal (HTM) ions in Tris-HCl/EtOH (7:3, v/v, pH 7.4). In addition, the turn-on fluorescent change upon the addition of Cu²⁺ is also applied in cell imaging.