Bilayer lipid membrane biosensor with enhanced stability for amperometric determination of hydrogen peroxide

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• 作者：Longzhen Zheng ; ^a ; ^{zlz@ecjtu.jx.cn"" rel=""nofollow} ; Leyan Xiong^a ; Dan Zheng^a ; Yindi Li^a ; Qiang Liu^a ; Kui Han^a ; Wen Liu^a ; Kun Tao^a ; Shaoming Yang^a ; Jian Xia^a
• 关键词：Polydopamine ; Electropolymerization ; Bilayer lipid membrane ; H₂O₂ biosensor ; Storage stability
• 刊名：Talanta
• 出版年：2011
• 出版时间：15 July 2011
• 年：2011
• 卷：85
• 期：1
• 页码：43-48
• 全文大小：782 K

文摘

In this paper, a polydopamine (PDA) film is electropolymerized on the surface of bilayer lipid membrane (BLM) which is immobilized with horseradish peroxidase (HRP). The coverage of the PDA film on HRP/BLM electrode is monitored by electrochemical impedance spectroscopy (EIS). The electrocatalytic reduction of H₂O₂ at the PDA/HRP/BLM electrode is studied by means of cyclic voltammetry (CV). The biosensor has a fast response to H₂O₂ of less than 5 s and an excellent linear relationship is obtained in the concentration range from 2.5 × 10⁻⁷ to 3.1 × 10⁻³ mol L⁻¹, with a detection limit of 1.0 × 10⁻⁷ mol L⁻¹ (S/N = 3). The response current of BLM/HRP/PDA biosensor retains 84 % of its original response after being stored in 0.1 mol L⁻¹ pH 7.0 PBS at 4 °C for 3 weeks. The selectivity, repeatability, and storage stability of PDA/HRP/BLM biosensor are greatly enhanced by the coverage of polydopamine film on BLM.