Bombyx mori cecropin A has a high antifungal activity to entomopathogenic fungus Beauveria bassiana
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- 作者：Dingding Lua ; b ; c ; Tao Genga ; b ; Chengxiang Houa ; b ; Yuxia Huanga ; b ; Guangxing Qina ; b ; Xijie Guoa ; b ; guoxijie@126.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：AMPs ; antimicrobial peptides ; BbHN6 ; Beauveria bassiana HN6 ; ORF ; open reading frame ; RNA ; ribonucleic acid ; DNA ; deoxyribonucleic acid ; cDNA ; complementary deoxyribonucleic acid ; IPTG ; isopropyl β-D-thiogalactoside ; OD ; optical density ; SDS-PAGE ; sodium dodecyl sulfate-polyacrylamide gel electrophoresis ; hpi ; hours post-inoculation ; LT50 ; the median lethal time ; PBS ; phosphate buffered saline ; PDA ; potato dextrose agar ; LB ; Luria&ndash ; Bertani ; PCR ; polymerase chain reaction ; RT-PCR ; reverse tr
- 刊名：Gene
- 出版年：2016
- 出版时间：25 May 2016
- 年：2016
- 卷：583
- 期：1
- 页码：29-35
- 全文大小：1309 K
文摘
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The expression of cecropin A gene was up-regulated in B. bassiana infected silkworm.
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Bombyx mori cecropin A was successfully expressed in E. coli and purified.
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BmCecA have a high antifungal activity to B. bassiana in vitro and in vivo.
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BmCecA plays important roles in silkworm innate defense against fungal infection.