A novel multicommuted fluorimetric optosensor for determination of resveratrol in beer

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• 作者：Luc&#xed ; a Molina-Garc&#xed ; a ; Antonio Ruiz-Medina ; Maria Luisa Ferná ; ndez-de Có ; rdova
• 关键词：Resveratrol ; Multicommutation ; Optosensor ; Photochemically induced fluorescence ; Beer
• 刊名：Talanta
• 出版年：2011
• 出版时间：15 January 2011
• 年：2011
• 卷：83
• 期：3
• 页码：850-856
• 全文大小：325 K

文摘

Resveratrol (3,5,4′-trihydroxystilbene) is a phytoalexin that plays a central role in the human diet because of its antioxidant, anticarcinogenic and antimutagenic properties. This paper shows the development of a multicommuted optosensing device for the determination of resveratrol in beer. The method is based on the measurement of the fluorescence (277/382 nm, λ_ex/λ_em) of the photoproduct on-line generated by UV-irradiation of resveratrol. The fluorescent photoproduct is monitored once it is retained on a solid support (Sephadex QAE A-25) in the detection area, which improves both sensitivity and selectivity. The sample was delipidated with toluene and cyclohexane and resveratrol was extracted by solid-phase extraction (SPE) on C₁₈ cartridges, using methanol as eluent. This pre-treatment allowed recovering about an 82 % resveratrol and removing its 3-O-β-d-glycoside (piceid) and other interfering substances present in beer. The method provides a detection limit (DL) of 1.0 ng mL⁻¹ and a linear dynamic range (LDR) of 3.3–100 ng mL⁻¹. It was satisfactorily applied to the determination of resveratrol in top- and bottom-fermented beers by standard addition calibration. Resveratrol concentrations in the analysed samples varied from 4.1 to 14.1 ng mL⁻¹. This is the first proposed spectroscopic method for determination of resveratrol in beer.