Development of europium doped core-shell silica cobalt ferrite functionalized nanoparticles for magnetic resonance imaging

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• 作者：Bhavesh D. Kevadiya^a ; Aditya N. Bade^a ; Christopher Woldstad^b ; Benson J. Edagwa^a ; JoEllyn M. McMillan^a ; Balasrinivasa R. Sajja^b ; Michael D. Boska^b ; Howard E. Gendelman^a ; ^{hegendel@unmc.edu}
• 关键词：Biodistribution ; Cobalt ferrite ; Multimodal imaging ; Nanoprobes ; Monocyte-macrophages ; Magnetic resonance imaging
• 刊名：Acta Biomaterialia
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：49
• 期：Complete
• 页码：507-520
• 全文大小：4664 K
• 卷排序：49

文摘

The size, shape and chemical composition of europium (Eu3+) cobalt ferrite (CFEu) nanoparticles were optimized for use as a “multimodal imaging nanoprobe” for combined fluorescence and magnetic resonance bioimaging. Doping Eu3+ ions into a CF structure imparts unique bioimaging and magnetic properties to the nanostructure that can be used for real-time screening of targeted nanoformulations for tissue biodistribution assessment. The CFEu nanoparticles (size ∼7.2 nm) were prepared by solvothermal techniques and encapsulated into poloxamer 407-coated mesoporous silica (Si-P407) to form superparamagnetic monodisperse Si-CFEu nanoparticles with a size of ∼140 nm. Folic acid (FA) nanoparticle decoration (FA-Si-CFEu, size ∼140 nm) facilitated monocyte-derived macrophage (MDM) targeting. FA-Si-CFEu MDM uptake and retention was higher than seen with Si-CFEu nanoparticles. The transverse relaxivity of both Si-CFEu and FA-Si-CFEu particles were r2 = 433.42 mM−1 s−1 and r2 = 419.52 mM−1 s−1 (in saline) and r2 = 736.57 mM−1 s−1 and r2 = 814.41 mM−1 s−1 (in MDM), respectively. The results were greater than a log order-of-magnitude than what was observed at replicate iron concentrations for ultrasmall superparamagnetic iron oxide (USPIO) particles (r2 = 31.15 mM−1 s−1 in saline) and paralleled data sets obtained for T2 magnetic resonance imaging. We now provide a developmental opportunity to employ these novel particles for theranostic drug distribution and efficacy evaluations.Statement of SignificanceA novel europium (Eu3+) doped cobalt ferrite (Si-CFEu) nanoparticle was produced for use as a bioimaging probe. Its notable multifunctional, fluorescence and imaging properties, allows rapid screening of future drug biodistribution. Decoration of the Si-CFEu particles with folic acid increased its sensitivity and specificity for magnetic resonance imaging over a more conventional ultrasmall superparamagnetic iron oxide particles. The future use of these particles in theranostic tests will serve as a platform for designing improved drug delivery strategies to combat inflammatory and infectious diseases.