Heregulin promotes expression and subcellular redistribution of ADP-ribosylation factor 3
详细信息 查看全文
- 作者：Li ; Feng ; Mandal ; Mahitosh ; Mishra ; Sandip K. ; Barnes ; Christopher J. ; Kumar ; Rakesh
- 关键词：ADP-ribosylation factor 3 ; Heregulin ; Breast cancer cell ; ARF3 ; ADP-ribosylation factor 3 ; HER ; human epidermal growth factor receptor ; HRG ; heregulin-β ; 1
- 刊名：FEBS Letters
- 出版年：2002
- 出版时间：July 31, 2002
- 年：2002
- 卷：524
- 期：1-3
- 页码：49-53
- 全文大小：282 K
文摘
To identify genes whose expression is modulated by heregulin-β1 (HRG), a regulatory polypeptide for mammary epithelial cells, we performed differential display screening of MCF-7 cell mRNA. One cDNA clone upregulated by HRG was identical to human ADP-ribosylation factor 3 (ARF3), a guanine nucleotide-binding protein functioning in vesicular trafficking, phospholipase D activation and intracellular transport. HRG treatment increased expression of ARF3 mRNA and protein. Also, HRG triggered a rapid redistribution of ARF3, first to cell membranes and then to the nuclear compartment, where ARF3 colocalized with acetylated histone H3 in discrete regions. In addition, the ARF3 protein was developmentally regulated in the mammary gland with the highest levels in virgin and post-weaning glands. Together, these findings suggest for the first time that stimulation of ARF3 expression, subcellular redistribution and interaction with acetylated histone H3 may play a role in the action of HRG in mammary epithelial cells.