An important role for adenine, cholera toxin, hydrocortisone and triiodothyronine in the proliferation, self-renewal and differentiation of limbal stem cells in vitro
详细信息 查看全文
- 作者：Min Yua ; 1 ; Sanja Bojica ; 1 ; Gustavo S. Figueiredoa ; d ; Paul Rooneyb ; Julian de Havillandc ; Anne Dickinsonc ; Francisco C. Figueiredoa ; d ; Majlinda Lakoa ; Majlinda.Lako@ncl.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Limbal stem cells ; Limbus ; Good manufacturing practice ; Adenine ; Cholera toxin ; Hydrocortisone ; Triiodothyronine
- 刊名：Experimental Eye Research
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：152
- 期：Complete
- 页码：113-122
- 全文大小：1901 K
文摘
- •
Adenine, cholera toxin, hydrocortisone and triiodothyronine are essential for LSCs proliferation and self-renewal.
- •
Adenine and triiodothyronine had a more profound impact as their removal led to LSC differentiation and loss of viability.
- •
Removal of each of four components from traditional culture media may pose a risk for clinical translations.
- •
New media composition in compliance with Good Manufacturing Practice is proposed.