NMR investigation of the isolated second voltage-sensing domain of human Nav1.4 channel

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• 作者：A.S. Paramonov^a ; ^b ; E.N. Lyukmanova^a ; ^b ; M.Yu. Myshkin^a ; ^c ; M.A. Shulepko^a ; ^b ; D.S. Kulbatskii^a ; ^b ; N.S. Petrosian^a ; ^c ; A.O. Chugunov^a ; D.A. Dolgikh^a ; ^b ; M.P. Kirpichnikov^a ; ^b ; A.S. Arseniev^a ; ^c ; Z.O. Shenkarev^a ; ^c ; ^{zakhar-shenkarev@yandex.ru}
• 关键词：CF ; cell-free ; DC7PC ; 1 ; 2-diheptanoyl-sn-glycero-3-phosphocholine ; DDM ; n-dodecyl β-d-maltoside ; DHPC ; 1 ; 2-dihexanoyl-sn-glycero-3-phosphocholine ; DMPC ; 1 ; 2-dimiristoyl-sn-glycero-3-phosphocholine ; DMPG ; 1 ; 2-dimiristoyl-sn-glycero-3-phosphoglycerol ; DOPG ; 1 ; 2-dioleoyl-sn-glycero-3-phosphoglycerol ; FM ; feeding mixture ; FOS-12 (DPC) ; n-dodecylphosphocholine ; FOS-14 ; n-tetradecylphosphocholine ; Kv channel ; K+ voltage-gated channel ; LDAO ; n-dodecyl-N ; N-dimethylamine-N-oxide ; LMPG ; 1-myristoyl-2-hydr
• 刊名：Biochimica et Biophysica Acta (BBA) - Biomembranes
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：1859
• 期：3
• 页码：493-506
• 全文大小：2807 K
• 卷排序：1859

文摘

Second voltage-sensing domain of human Nav1.4 channel (VSD-II) was studied by NMR VSD-II aggregates in environments containing phospholipids (bicelles, nanodiscs) Secondary structure of VSD-II in LPPG micelles is similar to bacterial Na+ channels μs-ms motions were observed in the regions of expected interhelical contacts of VSD ‘Divide and conquer’ approach is applicable for structural studies of Na+ channels