Comparative analysis of microbial DNA extraction protocols for groundwater samples

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• 作者：Jessica Purswani^a ; Antonio Manuel Martí ; n-Platero^b ; Patricia Reboleiro-Rivas^a ; Jes&#xfa ; s Gonzá ; lez-Ló ; pez^a ; ^b ; Clementina Pozo^a ; ^b ; ^{clpozo@ugr.es"" rel=""nofollow}
• 关键词：MSOP ; Chelex ; Epicentre ; FastDNA ; Fastprep ; DNA extraction ; TGGE ; Groundwater samples
• 刊名：Analytical Biochemistry
• 出版年：2011
• 出版时间：15 September 2011
• 年：2011
• 卷：416
• 期：2
• 页码：240-242
• 全文大小：239 K

文摘

A comparative analysis of four different DNA extraction protocols was performed to determine the best choice for groundwater microbial diversity studies using temperature gradient gel electrophoresis (TGGE) analysis. The methods used were a chelex-based method, a modified salting out procedure (MSOP), and the commercial kits Epicentre and FastDNA. Both commercial kits exhibited the greatest reproducibility in their methods; however, their band patterns were very different. The protocol that showed the highest diversity was the chelex-based method, and the one that showed the lowest diversity was the FastDNA kit.