Sensitivity of Chlamydia suis to cathelicidin peptides
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- 作者：Manuela Donati ; Antonietta Di Francesco ; Renato Gennaro ; Monica Benincasa ; Simone Magnino ; Salvatore Pignanelli ; Alisa Shurdhi ; Aless ; ra Moroni ; Claudio Mazzoni ; Giuseppe Merialdi ; et al.
- 关键词：Chlamydia suis ; Cathelicidin peptides
- 刊名：Veterinary Microbiology
- 出版年：2007
- 出版时间：20 July 2007
- 年：2007
- 卷：123
- 期：1-3
- 页码：269-273
- 全文大小：399 K
文摘
Nine Chlamydia suis isolates, obtained from pigs with conjunctivitis, were molecularly characterized by ompA sequencing and their in vitro susceptibility to six cathelicidin peptides (SMAP-29, BAC-7, BMAP-27, BMAP-27, BMAP-28, PG-1, LL-37) determined in cell culture. SMAP-29 was the most active peptide, reducing the intracellular inclusion number by ≥50 % at a concentration of 10 μg/ml (3 μM) in six of the nine isolates tested. Three molecularly identical isolates were insensitive at a concentration as high as 80 μg/ml (25 μM). Of the remaining cathelicidin peptides tested, BAC-7 and BMAP-27 were active against six C. suis isolates at a concentration of 80 μg/ml (25 and 26 μM, respectively). Cathelicidins LL-37 and PG-1 did not show any anti-chlamydial activity at 80 μg/ml.