Separation and identification of some common isomeric plant triterpenoids by thin-layer chromatography and high-performance liquid chromatography

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• 作者：Mitja Martelanc ; Irena Vovk ; Breda Simonovska
• 关键词：Triterpenoids ; Plant extracts ; Tomato ; Cabbage ; Rosemary ; Sage ; Oak ; Thin-layer chromatography ; Silica gel HPTLC ; C₁₈ RP-HPTLC ; HPLC-UV ; HPLC– ; APCI-MS– ; MS
• 刊名：Journal of Chromatography A
• 出版年：2009
• 出版时间：18 September 2009
• 年：2009
• 卷：1216
• 期：38
• 页码：6662-6670
• 全文大小：1199 K

文摘

Chromatographic separation of 10 triterpenoids (α-amyrin, β-amyrin, δ-amyrin, lupeol, lupenon, lupeol acetate, cycloartenol, cycloartenol acetate, ursolic acid, oleanolic acid) and 2 sterols (stigmasterol and β-sitosterol) was studied. The chromatographic techniques included silica gel and reversed-phase (C₁₈ RP) thin-layer chromatography (TLC) and C₁₈ RP high-performance liquid chromatography (HPLC) using UV and mass spectrometric (MS) detection with atmospheric pressure chemical ionization (APCI). The TLC separation of the isomeric triterpenols lupeol, α-amyrin, β-amyrin and cycloartenol was achieved for the first time using C₁₈ RP-HPTLC plates. Cycloartenol could be separated from related compounds only on C₁₈ RP-TLC but not on the C₁₈ RP-HPLC. δ-Amyrin isolated from the tomato fruit surface extract could be separated from other amyrins only by HPLC. Tandem mass spectrometry allowed discrimination between the isomers lupeol, α-amyrin, β-amyrin, δ-amyrin, cycloartenol and between lupeol acetate and cycloartenol acetate. The combination of 3 TLC methods and 2 HPLC methods enables qualitative determination of all 12 compounds and proves to be useful for the analysis of plant extracts. It is recommended that TLC screening on silica gel and C₁₈ RP be performed before HPLC analysis.