Co-infusion of donor bone marrow with host mesenchymal stem cells treats GVHD and promotes vascularized skin allograft survival in rats

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• 作者：Ali Emre Aksu ; Elaine Horibe ; Justin Sacks ; Ryosuke Ikeguchi ; Jeremy Breitinger ; Merissa Scozio ; Jignesh Unadkat ; Maryam Feili-Hariri
• 关键词：Rat ; Mesenchymal stem cells ; Bone marrow ; GVHD ; Vascularized skin
• 刊名：Clinical Immunology
• 出版年：2008
• 出版时间：June 2008
• 年：2008
• 卷：127
• 期：3
• 页码：348-358
• 全文大小：1368 K

文摘

We investigated the effect of autologous mesenchymal stem cells (MSC) on multiple unmodified donor bone marrow (BM) infusions and vascularized skin graft outcome. BM-derived rat MSC were examined for phenotype and function. MSC/MSC-conditioned-medium suppressed IFN-γ production by T cells and modified DC function. Infusions of MSC with one-time BM improved vascularized skin graft survival, while with one–two-times BM reversed graft versus host disease (GVHD). Mixed chimerism was enhanced in recipients given two–four-times BM with MSC infusions. Interestingly, four-times BM infusions with MSC delayed GVHD onset, reduced host tissue damage and enhanced vascularized skin allograft survival compared to four-times BM alone. These data demonstrate that, the co-infusion of MSC with unmodified BM limit the toxicity of allogeneic BM transplantation, enhance mixed chimerism and improve vascularized skin graft survival. These findings provide insights for the development of autologous MSC-based BM transplantation and prevention of graft rejection or treatment of autoimmunity.