Role of the liver in determining alloimmune response in vitro following donor-specific spleen cell injection
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- 作者：Masaki Okuyama ; Hiroaki Nagano ; Shogo Kobayashi ; Li He ; Hirofumi Ota ; Junzo Shimizu ; Yutaka Takeda ; Shigeru Masrubashi ; Hidetoshi Eguchi ; Masahiro Tanemuea ; Keizo Dono ; Masato Sakon ; Koji Umeshita ; Mi
- 关键词：Alloantigen ; DSI ; Rejection ; Transplantation ; Liver ; Spleen
- 刊名：Transplant Immunology
- 出版年：2010
- 出版时间：February 2010
- 年：2010
- 卷：22
- 期：3-4
- 页码：150-156
- 全文大小：764 K
文摘
The aim of our study was to investigate the allogeneic influence inside and outside the liver in vitro following donor-specific cell injection (DSI). DA rats (RT1a) were used as donors and WS rats (RT1k) as recipients. WS were sensitized with DA spleen cells, followed 24 h later by total hepatectomy. The liver was transplanted into another WS (sensitized liver-grafted; SL-Grafted). The hepatectomized WS underwent liver transplantation from a naive WS (sensitized liver-removed; SL-Removed). Alloantigens accumulated in the liver in SL-Grafted and in the extrahepatic tissue/organ(s) in SL-Removed. DA hearts were transplanted 10 days after antigen administration. To analyze the immune responses, we measured Th1/Th2 cytokine profiles, and perforin mRNA in various organs, allogeneic mixed lymphocyte reaction (MLR), and donor-specific immunoglobulin. Th1 cytokine levels in the liver of SL-Grafted and in spleen of SL-Removed were highly and rapidly upregulated but decreased thereafter. IFN-γ and perforin mRNAs were significantly higher in SL-Grafted and lower in SL-Removed. MLR was significantly higher in SL-Grafted than SL-Removed and controls. There was no significant difference in the donor-specific immunoglobulin level. Our findings suggest that liver and other organs may behave differently to alloantigen, suggesting the importance of an early Th1 reaction in the liver and spleen.