URB expression in human bone marrow stromal cells and during mouse development
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- 作者：Liu ; Yi ; Monticone ; Massimiliano ; Tonachini ; Laura ; Mastrogiacomo ; Maddalena ; Marigo ; Valeria ; et. al.
- 关键词：BMSC ; Cartilage ; Extracellular matrix ; Hypertrophic chondrocyte ; Skeletogenesis ; Sushi-repeat-containing protein ; Urb
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2004
- 出版时间：September 17, 2004
- 年：2004
- 卷：322
- 期：2
- 页码：497-507
- 全文大小：2.84 M
文摘
Seven genes preferentially expressed in undifferentiated human bone marrow stromal cells (BMSC) with respect to BMSC-derived osteoblasts were previously identified by differential display. Here we characterize the expression of one of these genes, URB, belonging to the sushi-repeat-containing protein superfamily. In culture, URB is expressed in both human primary and cloned BMSC, and is drastically downregulated during osteoblastic differentiation of these cells. Here we show that in mouse tissues a single 3.8kb Urb transcript is detected and that the mouse Urb protein is secreted as a 150kDa glycoprotein. During mouse development Urb RNA is barely detectable in 9dpc embryos and increases at later stages. Both in situ hybridization and immunohistochemistry analysis show Urb expression in mouse embryos starting from 14dpc mostly in cartilage. The temporal and spatial expression pattern of Urb suggests its role in mouse skeletogenesis.