Comparison of different approaches to quantitative adenovirus detection in stool specimens of hematopoietic stem cell transplant recipients

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• 作者：K. Kosulin^a ; S. Dworzak^a ; A. Lawitschka^b ; S. Matthes-Leodolter^b ; T. Lion^a ; ^c ; ^{thomas.lion@ccri.at}
• 关键词：HAdV ; human adenovirus ; allo-HSCT ; allogeneic hematopoietic stem cell transplantation ; PhHV ; phocine herpesvirus ; IC2 ; internal control (Adenovirus R-gene PCR Kit bioMé ; rieux)
• 刊名：Journal of Clinical Virology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：85
• 期：Complete
• 页码：31-36
• 全文大小：734 K
• 卷排序：85

文摘

Standardized adenovirus DNA isolation/qPCR is necessary for successful theranostics. Different DNA isolations have more influence on quantitative virus analysis than PCRs. Amplification efficiencies differ for adenoviral species by different PCR assays. Dynamic range is between 103 and 108 copies/g stool for both PCR assays.