CHO microRNA engineering is growing up: Recent successes and future challenges

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• 作者：Vaibhav Jadhav ; Matthias Hackl ; Aliaks ; r Druz ; Smriti Shridhar ; Cheng-Yu Chung ; Kelley M. Heffner ; David P. Kreil ; Mike Betenbaugh ; Joseph Shiloach ; Niall Barron ; Johannes Grillari ; Nicole Borth
• 关键词：MicroRNA engineering ; Chinese Hamster Ovary cells ; Bioprocess relevant properties
• 刊名：Biotechnology Advances
• 出版年：December, 2013
• 年：2013
• 卷：31
• 期：8
• 页码：1501-1513
• 全文大小：1040 K

文摘

microRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines.