Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry
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- 作者：Bo Tiana ; Zhen Qiua ; Jing Mab ; Teresa Zardá ; n Gó ; mez de la Torrea ; Christer Johanssonc ; Peter Svedlindha ; Mattias Strö ; mberga ; mattias.stromberg@angstrom.uu.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：Zika virus ; Loop-mediated isothermal amplification ; Magnetic nanoparticles ; Brownian relaxation ; AC susceptometer
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：420-425
- 全文大小：1535 K
文摘
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Synthetic Zika virus oligonucleotide is quantified by an AC susceptometer.
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Amplicons of the LAMP reaction are attached to the magnetic nanoparticles.
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Mg2P2O7 deposits onto the nanoparticles and further improves the signal.
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An LOD of 1 aM oligos can be achieved with a total assay time of 27 min.