Effects of Melatonin on Liver Estrogen Receptor and Vitellogenin Expression in Rainbow Trout: An in Vitro and in Vivo Study

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• 作者：Mazurais ; David ; Porter ; Mark ; Lethimonier ; Christè ; le ; Le Dré ; an ; Gwenola ; Le Goff ; Pascale ; et. al.
• 刊名：General and Comparative Endocrinology
• 出版年：2000
• 出版时间：May, 2000
• 年：2000
• 卷：118
• 期：2
• 页码：344-353
• 全文大小：99.3 K

文摘

Although melatonin is believed to mediate many seasonal and circadian effects of photoperiod on reproduction in salmonids, the precise mechanisms underlying such effects are still largely unknown. Recent data of the literature indicate a relationship between melatonin and expression of estrogen receptors (ER) in various tissues. In this study, the effects of melatonin on estrogen receptor and/or vitellogenin expression were studied by a combination of in vivo and in vitro experiments. In yeast stably expressing ER and transfected with an estrogen-responsive element-β-galactosidase reporter gene, melatonin had no effect on basal or E2-stimulated ER expression. Incubation of hepatocyte aggregates with melatonin (10⁻⁸ to 10⁻⁴) for 16 or 48 h did not modify the E2-stimulated ER and vitellogenin mRNA, as measured by dot blots. Finally, neither pinealectomy nor melatonin implants caused any effect on basal or E2-stimulated ER and vitellogenin mRNA contents in the liver. Altogether, these results suggest that, athough we cannot exclude potential effects at the brain or pituitary levels, melatonin has no or little effects on estrogen receptor in the liver.