Analysis of a temperature-sensitive mutation in Uba1: Effects of the click reaction on subsequent immunolabeling of proteins involved in DNA replication
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- 作者：Kimihiko Sugaya ; k_sugaya@nirs.go.jp" class="auth_mail" title="E-mail the corresponding author ; Yoshie Ishihara ; Sonoe Inoue
- 关键词：BrdU ; 5-bromo-2&prime ; -deoxyuridine ; CldU ; 5-chloro-2&prime ; -deoxyuridine ; EdU ; 5-ethynyl-2&prime ; -deoxyuridine ; E1 ; ubiquitin activating enzyme ; E2 ; ubiquitin conjugating enzyme ; E3 ; ubiquitin ligase ; DIG-dUTP ; digoxigenin-dUTP ; IdU ; 5-iodo-2&prime ; -deoxyuridine ; MCM7 ; mini-chromosome maintenance protein 7 ; PCNA ; proliferating cell nuclear antigen ; PFA ; paraformaldehyde ; ts ; temperature-sensitive
- 刊名：FEBS Open Bio
- 出版年：2015
- 出版时间：2015
- 年：2015
- 卷：5
- 期：Complete
- 页码：167-174
- 全文大小：2321 K
文摘
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The click reaction inhibits the indirect immunolabeling of Cdt1.
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The click reaction allows for the detection of PCNA with nascent DNA.
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Cdt1 appears to remain close to replication sites in the ts-mutant of Uba1.
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Impaired ubiquitination caused by the defect of Uba1 affects DNA replication only slowly.