Establishment and Characterization of Three Malacosoma disstria Cell Lines
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文摘
Three cell lines were established from Malacosoma disstria hemocytes. Although the morphology, growth rates, isozyme patterns, and adherence characteristics of these cell lines (UA-Md203, UA-Md210, and UA-Md221) were different, all supported virus replication (AcMNPV and MdMNPV-A92). One cell line, UA-Md221 was extremely adherent and could not be removed by enzymatic treatment. Few cells were loosened or damaged by 2-hr incubations in trypsin, collagenase or enzyme mixture. We hypothesize that these cells inactivate these enzymes.