Polyoxometalates as peroxidase mimetics and their applications in H₂O₂ and glucose detection

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• 作者：Jingjing Wang^a ; Dongxue Han^b ; Xiaohong Wang^a ; ^{wangxh665@nenu.edu.cn} ; ^{wxh.wangxiaohong665@yahoo.com.cn} ; Bin Qi^a ; ; Meisheng Zhao^c ; ^{zhaoms1961@sina.com}
• 关键词：Polyoxometalates ; Peroxidase-like ; Colorimetric detection ; H2O2 ; Glucose
• 刊名：Biosensors and Bioelectronics
• 出版年：2012
• 出版时间：June-July, 2012
• 年：2012
• 卷：36
• 期：1
• 页码：18-21
• 全文大小：326 K

文摘

Polyoxometalates (H₃PW₁₂O₄₀, H₄SiW₁₂O₄₀ and H₃PMo₁₂O₄₀) have been proven to possess intrinsic peroxidase-like activity for the first time, which can catalyze oxidation of the peroxidase substrate 3,3?5,5?tetramethylbenzidine (TMB) by H₂O₂ to form a blue color in aqueous solution. Among them, H₃PW₁₂O₄₀ (PW₁₂) exhibits higher catalytic activity to TMB than natural enzyme HRP and other two POMs. In addition, H₃PW₁₂O₄₀/graphene exhibited higher activity than H₃PW₁₂O₄₀ in this catalytic oxidation reaction due to the effect of graphene in promoting the electron transfer between the substrate and catalyst. POMs/H₂O₂/TMB system provides a simple, accurate approach to colorimetric detection for H₂O₂ or glucose. The colorimetric method based on POMs showed good response toward H₂O₂ and glucose detection with a linear range from 1.34¡Á10^? to 6.7¡Á10^? mol/L and 1¡Á10^? to 1¡Á10^? mol/L, respectively. The results showed that it is a simple, cheap, more convenient, highly selective, sensitive, and easy handling colorimetric assay.