Determination of urinary biogenic amines’ biomarker profile in neuroblastoma and pheochromocytoma patients by MEKC method with preceding dispersive liquid-liquid microextraction
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- 作者：Natalia Miękusa ; Ilona Olędzkab ; Alina Plenisb ; Piotr Kowalskib ; Ewa Bieńc ; Aleksandra Miękusc ; Małgorzata Anna Krawczykc ; Elżbieta Adamkiewicz-Drożyńskac ; Tomasz Bączekb ; tbaczek@gumed.edu.pl" class="auth_mail" title="E-mail the corresponding author
- 关键词：Neuroblastoma ; Biogenic amines (BAs) ; Cancer biomarkers ; Urine sample analysis ; Micellar electrokinetic chromatography (MEKC) ; Dispersive liquid&ndash ; liquid microextraction (DLLME) ; Hierarchical Cluster Analysis (HCA)
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：1036-1037
- 期：Complete
- 页码：114-123
- 全文大小：1482 K
文摘
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The optimization of the method of extraction of seven compounds from among BAs and their precursors from urine samples.
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Dispersive liquid-liquid microextraction (DLLME) has been chosen for its high efficiency of isolation and clean-up of biological matrix.
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α-cyclodextrin-modified micellar electrokinetic chromatography has been applied for quantification of the analyzed compounds.
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The DLLME-MEKC-UV method for BAs analysis could be successfully applied in clinical practice to confirm the diagnosis of neuroendocrine tumors.